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Publication Citation: Determining carbapenemase activity with 18O labeling and targeted mass spectrometry

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Author(s): Meiyao Wang; Yang Shen; Illarion V. Turko; Daniel Nelson; Shuwei Li;
Title: Determining carbapenemase activity with 18O labeling and targeted mass spectrometry
Published: October 16, 2013
Abstract: Carbapenems are broad spectrum antibiotics considered as last resort drugs to treat bacterial infections. Carbapenem-hydrolyzing β-lactamases (also called carbapenemases), however, can confer bacterial resistance and represent a serious health threat. Here, we report a novel approach using 18O labeling and selected reaction monitoring to detect carbapenemase activity from pathogenic microorganisms in a rapid and quantitative manner. Four model bacterial strains bearing various classes of β-lactamases were tested for their capability to hydrolyze meropenem, a FDA-approved carbapenem drug. We were able to predict the meropenem resistance of these bacteria based upon their carbapenemase activity, suggesting the great potential of our method in clinical diagnostics.
Citation: Analytical Chemistry
Volume: 85
Issue: 22
Pages: pp. 11014 - 11019
Keywords: carbapenemase; selected reaction monitoring; isotope labelling; mass spectrometry
Research Areas: Diagnostics, Drugs/Pharmaceuticals, Bioscience & Health
DOI: http://dx.doi.org/10.1021/ac402627k  (Note: May link to a non-U.S. Government webpage)