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Publication Citation: Single molecule confocal fluorescence lifetime correlation spectroscopy for accurate nanoparticle size determination

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Author(s): Bonghwan Chon; Kimberly A. Briggman; Jeeseong Hwang;
Title: Single molecule confocal fluorescence lifetime correlation spectroscopy for accurate nanoparticle size determination
Published: May 15, 2014
Abstract: We report on an experimental procedure in confocal single molecule fluorescence lifetime correlation spectroscopy (FLCS) to determine the range of excitation power and molecule concentration in solution under which the application of an unmodified model autocorrelation function is justified. This procedure enables fitting of the autocorrelation to an accurate model to measure diffusion length (r) and diffusion time (τD) of single molecules in solution. We also report on the pinhole size dependency of r and τD in a confocal FLCS platform. This procedure determines a set of experimental parameters with which the Stoke-Einstein equation accurately measures the hydrodynamic radii of spherical nanoparticles, enabling the determination of the particle size range for which the hydrodynamic radius by the S-E equation measures the real particle radius.
Citation: Physical Chemistry Chemical Physics
Volume: 16
Pages: pp. 13418 - 13425
Keywords: single molecule;Fluorescence correlation spectroscopy;FCS; nanoparticle;fluorescence lifetime; confocal microscopy;Fluorescence lifetime correlation spectroscopy;FLCS
Research Areas: Optical Metrology, Nanotech/Environment, Health & Safety, Particle, Optical microscopy, Single Molecule Biophysics, Nanotechnology, Characterization, Nanometrology, and Nanoscale Measurements
DOI: http://dx.doi.org/10.1039/C4CP01197J  (Note: May link to a non-U.S. Government webpage)