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Publication Citation: Optimal Laser Scan Path for Localizing a Fluorescent Particle in Two or Three Dimensions

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Author(s): Gregg M. Gallatin; Andrew J. Berglund;
Title: Optimal Laser Scan Path for Localizing a Fluorescent Particle in Two or Three Dimensions
Published: July 16, 2012
Abstract: Localizing a fluorescent particle by scanning a focused laser in its vicinity and analyzing the detected photon stream provides real-time information for a modern class of feedback control systems for particle tracking and trapping. We present a simple mathematical test to determine whether a particular laser scan path encodes maximal information about a fluorescent particle's position in the detected photon stream. We give the optimal path for a Gaussian laser scanned in two or three dimensions, and present the Cram\'{e}r-Rao bound on the two and three-dimensional localization accuracy that can be achieved by analyzing the detected photon stream.
Citation: Optics Express
Volume: 20
Issue: 15
Pages: pp. 16381 - 16393
Keywords: single particle tracking
Research Areas: Optics, Optical microscopy
PDF version: PDF Document Click here to retrieve PDF version of paper (862KB)