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|Author(s):||Margaret C. Kline; Carolyn R. Steffen; Jamie L. Almeida; Erica L. Romsos; Michael D. Coble; John M. Butler;|
|Title:||The Latest and Greatest NIST PCR-based DNA Profiling Standard: Updates and Status of Candidate Standard Reference Material (SRM) 2391c|
|Published:||November 01, 2011|
|Abstract:||Standard Reference Material 2391c (SRM 2391c) PCR-based DNA Profiling Standard is the third renewal of this SRM, originally released in 1995. SRM 2391c consists of 6 candidate components labeled A through F. Components A through D are supplied as genomic DNA solutions packaged in perfluoroalkoxy fluoropolymer (PFA) vials (with component A as a single source female; component B as a single source male; component C as a single source male: and component D as a mixture of 3 parts components A and 1 part component C. Components E (single source female) and F (single source male) are cells (=75,000 cells per spot) deposited on 903 and FTA papers respectively, two 6 mm punches per component. Inclusion of two kinds of storage paper will enable laboratores to test direct PCR methods.|
|Citation:||No Journal Selected|
|Research Areas:||Bioscience & Health|
|DOI:||http://dx.doi.org/http://www.promega.com/resources/articles/profiles (Note: May link to a non-U.S. Government webpage)|