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|Author(s):||Hyeong G. Kang; Fuyuki Tokumasu; Matthew L. Clarke; Zhenping Zhou; Jianyong Tang; Tinh Nguyen; Jeeseong Hwang;|
|Title:||Probing Dynamic Fluorescence Properties of Single and Clustered Quantum Dots Towards Quantitative Biomedical Imaging of Cells|
|Published:||October 28, 2009|
|Abstract:||We present results on dynamical fluorescence properties of bioconjugated nanocrystals or quantum dots (QDs) in different chemical and physical environments. A variety of QD samples were prepared and compared: isolated individual QDs, QDs aggregated into clusters, and QDs conjugated to other nanoscale materials such as single walled carbon nanotubes and proteins in the plasma membrane of human erythrocytes. We discuss plausible scenarios to explain the results obtained for the fluorescence characteristics of QDs in these samples especially for the excitation time dependent fluorescence emission from clustered QDs. We also qualitatively demonstrate enhanced fluorescence emission signal from clustered QDs and deduce that the band 3 membrane proteins in erythrocyte membranes are clustered. This approach is promising for the development of QD-based quantitative molecular imaging techniques for biomedical studies involving clustering of biomolecules.|
|Citation:||Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology|
|Pages:||pp. 48 - 58|
|Keywords:||Quantum dot, Nano-assembly, Fluorescent nanoparticle, Protein oligomerization, Cell imaging|
|PDF version:||Click here to retrieve PDF version of paper (555KB)|