Differences in PDMS Modification Affect Laminin Deposition and Smooth Muscle Cell Response

Published

August 16, 2009

Author(s)

Joy P. Dunkers, Hae-Jeong Lee, Marvi Matos, Lisa Pakstis, Juan M. Taboas, Steven D. Hudson, Marcus T. Cicerone

Abstract

When culturing cells on flexible surfaces, it is important to consider extracellular matrix treatments that will remain on the surface under mechanical strain. Here we investigate differences in laminin surfaces that were deposited on oxidized PDMS (plasma only) versus oxidized PDMS treated with aminopropyltrimethoxysilane (silane-linked). We use specular X-ray reflectivity (SXR), transmission electron microscopy (TEM), and immunofluorescence to probe the quantity and uniformity of the laminin on the treated PDMS surfaces. We compare vascular smooth muscle cell (SMC) proliferation on laminin under equibiaxial strain between plasma only and silane-linked treatments.

Proceedings Title

American Chemical Society Division of Polymeric Materials: Science and Engineering Meeting| |Proceedings of the ACS Division of Polymeric Materials: Science & Engineering |ACS

Volume

101

Conference Dates

August 16-20, 2009

Conference Location

Washingont, DC

Pub Type

Conferences

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Local Download

Keywords

specular X-ray reflectivity, transmission electron microscopy, laminin, PDMS, smooth muscle cell

Citation

Dunkers, J. , Lee, H. , Matos, M. , Pakstis, L. , Taboas, J. , Hudson, S. and Cicerone, M. (2009), Differences in PDMS Modification Affect Laminin Deposition and Smooth Muscle Cell Response, American Chemical Society Division of Polymeric Materials: Science and Engineering Meeting| |Proceedings of the ACS Division of Polymeric Materials: Science & Engineering |ACS, Washingont, DC, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=901800 (Accessed April 18, 2024)