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|Author(s):||Joy P. Dunkers; Juan M. Taboas;|
|Title:||Response of Vascular Smooth Muscle Cells Under Mechanical Deformation Using Silane-Linked Laminin|
|Published:||April 22, 2009|
|Abstract:||For mechanotransduction studies, extracellular matrix proteins should be robustly attached to the surface to prevent cell delamination during deformation. The standard surface modification method is to incubate proteins on an oxidized, flexible surface (i.e. polydimethylsiloxane (PDMS)) without any additional surface modification. This results in physically adsorbed surface proteins that desorb over time even under static conditions. Cell response on surfaces with covalently bound proteins is often different from physical adsorption due to protein conformation alteration. We have found that silane-linked proteins give the highest coverage, uniformity, and cell proliferation over physically adsorbed and covalently bonded proteins. In this work, we evaluate the robustness of physically adsorbed and silane-linked laminin and proliferation of smooth muscle cells after equibiaxial stretching for 4 d.|
|Proceedings:||Society for Biomaterials Annual Meeting and Exposition|
|Location:||San Antonio, TX|
|Dates:||April 22-25, 2009|
|Keywords:||smooth muscle cells, laminin, mechanostransduction, silane, flexcell|
|Research Areas:||Materials Science, Characterization|