NIST logo

Publication Citation: Response of Vascular Smooth Muscle Cells Under Mechanical Deformation Using Silane-Linked Laminin

NIST Authors in Bold

Author(s): Joy P. Dunkers; Juan M. Taboas;
Title: Response of Vascular Smooth Muscle Cells Under Mechanical Deformation Using Silane-Linked Laminin
Published: April 22, 2009
Abstract: For mechanotransduction studies, extracellular matrix proteins should be robustly attached to the surface to prevent cell delamination during deformation. The standard surface modification method is to incubate proteins on an oxidized, flexible surface (i.e. polydimethylsiloxane (PDMS)) without any additional surface modification. This results in physically adsorbed surface proteins that desorb over time even under static conditions. Cell response on surfaces with covalently bound proteins is often different from physical adsorption due to protein conformation alteration. We have found that silane-linked proteins give the highest coverage, uniformity, and cell proliferation over physically adsorbed and covalently bonded proteins. In this work, we evaluate the robustness of physically adsorbed and silane-linked laminin and proliferation of smooth muscle cells after equibiaxial stretching for 4 d.
Proceedings: Society for Biomaterials Annual Meeting and Exposition
Location: San Antonio, TX
Dates: April 22-25, 2009
Keywords: smooth muscle cells; laminin; mechanostransduction, silane, flexcell
Research Areas: Characterization, Materials Science