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|Author(s):||J R. Krogmeier; Hyeong G. Kang; Matthew L. Clarke; p Yim; Jeeseong Hwang;|
|Title:||Probing the dynamic fluorescence properties of single water-soluble quantum dots|
|Published:||April 01, 2008|
|Abstract:||The influence of surface modifications, including carboxyl-, amine-, and mercaptoundecanoicacid-functionalized chemistries, on the dynamic fluorescence properties of single quantum dots (QDs) are probed using a combined confocal fluorescence microscopy and spectroscopy system. Water-soluble QDs with carboxyl- or amine-functionalized surfaces prepared through a linker arm show significant fluorescence intermittency and appear statistically identical. In contrast, water-soluble QDs functionalized via mercaptoundecanoicacid, an electron-donating thiol group, demonstrate suppressed fluorescence intermittency suggesting the photostability of water-soluble QDs is strongly dependant upon the method of surface functionalization. In addition, the long-term photostability of the mercaptoundecanoicacid-functionalized QD demonstrates an excitation-time-dependent blue-shift in the fluorescence emission spectra. Our measurement and analysis techniques promise a wealth of insight in elucidating the photophysics of bio-conjugated QDs and assessing and designing bio-conjugated QDs for biomedical applications requiring long-term photostabililty.|
|Pages:||pp. 1781 - 1788|
|Keywords:||bio-nanotechnology,confocal microscope,exciton,fluorescence,nanocrystal,single quantum dot,water-soluble quantum dot|