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Publication Citation: Microfluidic Temperature Gradient Focusing

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Author(s): David J. Ross; Laurie E. Locascio;
Title: Microfluidic Temperature Gradient Focusing
Published: June 01, 2002
Abstract: A new technique is described for the concentration and separation of ionic species in a microchannels. Concentration is achieved by balancing the electrophoretic velocity of an analyte against the bulk flow of solution in the presence of a temperature gradient. With an appropriate buffer, the temperature gradient can generate a corresponding gradient in the electrophoretic velocity, so that the electrophoretic and bulk velocities sum to zero at a unique point, resulting in analyte focusing. The technique for a variety of analytes including, amino acids, DNA, proteins, and particles, is shown to be capable of greater than 10000-fold concentration.
Citation: Analytical Chemistry
Volume: 74
Issue: No. 11
Pages: pp. 2556 - 2564
Keywords: focusing,microfluidics,preconcentration
Research Areas: