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NIST Authors in Bold
|Author(s):||David J. Ross; Laurie E. Locascio;|
|Title:||Microfluidic Temperature Gradient Focusing|
|Published:||June 01, 2002|
|Abstract:||A new technique is described for the concentration and separation of ionic species in a microchannels. Concentration is achieved by balancing the electrophoretic velocity of an analyte against the bulk flow of solution in the presence of a temperature gradient. With an appropriate buffer, the temperature gradient can generate a corresponding gradient in the electrophoretic velocity, so that the electrophoretic and bulk velocities sum to zero at a unique point, resulting in analyte focusing. The technique for a variety of analytes including, amino acids, DNA, proteins, and particles, is shown to be capable of greater than 10000-fold concentration.|
|Pages:||pp. 2556 - 2564|