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|Author(s):||Peter M. Vallone; J E. Devaney; J M. Marino;|
|Title:||A Strategy for Examining Complex Mixtures of Deoxyoligonucleotides Using IP-RP HPLC, MALDI-TOF MD, and Informatics|
|Published:||March 17, 2002|
|Abstract:||Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)and ion pair-reverse phase high performance liquid chromatography (IP-RP HPLC) techniques were combined to determine the sequence identity of short single-stranded deoxyoligonucleotides. In demonstrating this methodology a commercially abailable multiplex set of 8 primer pairs used for amplifying 8 specific loci, but with unknown sequences were separated and collected by IP-RP HPLC. Partial sequence information for IP-RP HPLC fractions was obtained from analyzing exonuclease digestion products by MALDI-TOF MS. By utilizing IP-RP HPLC, MALDI-TOF MS, exonuclease digests, and a simple computational algorithm an integrated strategy for determining the sequence of short necleic acid oligomers is demonstrated.|
|Pages:||pp. 257 - 265|
|Keywords:||informatics,MALDI-TOF MS,nucleic acid,PCR|
|Research Areas:||Forensics, DNA|