| Author(s): |
Peter M. Vallone; J E. Devaney; J M. Marino; |
| Title: |
A Strategy for Examining Complex Mixtures of Deoxyoligonucleotides Using IP-RP HPLC, MALDI-TOF MD, and Informatics |
| Published: |
March 17, 2002 |
| Abstract: |
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)and ion pair-reverse phase high performance liquid chromatography (IP-RP HPLC) techniques were combined to determine the sequence identity of short single-stranded deoxyoligonucleotides. In demonstrating this methodology a commercially abailable multiplex set of 8 primer pairs used for amplifying 8 specific loci, but with unknown sequences were separated and collected by IP-RP HPLC. Partial sequence information for IP-RP HPLC fractions was obtained from analyzing exonuclease digestion products by MALDI-TOF MS. By utilizing IP-RP HPLC, MALDI-TOF MS, exonuclease digests, and a simple computational algorithm an integrated strategy for determining the sequence of short necleic acid oligomers is demonstrated. |
| Citation: |
Analytical Biochemistry |
| Volume: |
304 |
| Pages: |
pp. 257 - 265 |
| Keywords: |
informatics;MALDI-TOF MS;nucleic acid;PCR |
| Research Areas: |
Forensics, DNA |
