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Publication Citation: A Uniaxial bioMEMS device for imaging single cell response during quantitative force-displacement measurements

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Author(s): David Serrell; Jera Law; Andrew J. Slifka; Roop L. Mahajan; Dudley S. Finch;
Title: A Uniaxial bioMEMS device for imaging single cell response during quantitative force-displacement measurements
Published: December 01, 2008
Abstract: Mechanical forces play a key role in many cellular processes such as apoptosis and differentiation. A microfabricated device has been developed for imaging of a single, adherent cell while quantifying force under an applied strain. The device works in a similar fashion to a displacement-controlled uniaxial tensile machine. The device was calibrated using a tipless AFM cantilever and shows excellent agreement with the calculated spring constant. A step input was applied to a single, adherent fibroblast cell and the viscoelastic response was characterized with a mechanical model. The adherent fibroblast was imaged using fluorescence and phase contrast techniques.
Citation: Biomedical Microdevices
Volume: 6
Pages: pp. 883 - 889
Keywords: bioMEMS;cell mechanics, fibroblast
Research Areas: Medical Devices
PDF version: PDF Document Click here to retrieve PDF version of paper (338KB)