| Author(s): |
David Serrell; Jera Law; Andrew J. Slifka; Roop L. Mahajan; Dudley S. Finch; |
| Title: |
A Uniaxial bioMEMS device for imaging single cell response during quantitative force-displacement measurements |
| Published: |
December 01, 2008 |
| Abstract: |
Mechanical forces play a key role in many cellular processes such as apoptosis and differentiation. A microfabricated device has been developed for imaging of a single, adherent cell while quantifying force under an applied strain. The device works in a similar fashion to a displacement-controlled uniaxial tensile machine. The device was calibrated using a tipless AFM cantilever and shows excellent agreement with the calculated spring constant. A step input was applied to a single, adherent fibroblast cell and the viscoelastic response was characterized with a mechanical model. The adherent fibroblast was imaged using fluorescence and phase contrast techniques. |
| Citation: |
Biomedical Microdevices |
| Volume: |
6 |
| Pages: |
pp. 883 - 889 |
| Keywords: |
bioMEMS;cell mechanics, fibroblast |
| Research Areas: |
Medical Devices |
| PDF version: |
 Click here to retrieve PDF version of paper (330KB) |
