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Publication Citation: Precise Characterization of Liposome Populations Formed in a 5-Channel Flow-Focused System

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Author(s): Andreas Jahn; Wyatt N. Vreeland; Don DeVoe; Laurie E. Locascio; Michael Gaitan;
Title: Precise Characterization of Liposome Populations Formed in a 5-Channel Flow-Focused System
Published: November 09, 2006
Abstract: Previously, we demonstrated liposome formation using hydrodynamic focusing in a microfluidic network which enables us to produce liposomes of a controlled size and size distribution in a reproducible fashion [1]. Former measurements on liposomes were conducted with batch dynamic light scattering (DLS) and multi angle laser light scattering (MALLS) of unfractionated samples. However, this measurement only provides ensemble averages. In this paper, a more detailed and accurate measurement technique that allows for precise characterization of liposome populations using asymmetric flow field flow fractionation (AFFFF) before MALLS and DLS is shown. Details about liposome size and size distribution allow further elucidation about the liposome self-assembly process.
Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (?TAS2006)
Proceedings: Proceedings of the 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (?TAS2006)
Pages: pp. 119 - 121
Location: Tokyo, JA
Dates: November 5-9, 2006
Keywords: flow field flow fractionation;liposomes;nanoparticle;self-assembly
Research Areas: