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Publication Citation: Photopatterned Polyelectrolyte Multilayers for Cell Culture

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Author(s): Darwin R. Reyes-Hernandez; J. Brian Hutchison; Laurie E. Locascio; Michael Gaitan;
Title: Photopatterned Polyelectrolyte Multilayers for Cell Culture
Published: November 09, 2006
Abstract: Patterned two-dimensional cell cultures are increasingly important for constructing bioanalytical tools for cell and molecular biology to address questions about interactions, signaling, migration, and differentiation, among others. This contribution presents a unique method for patterning layer-by- layer polymer with dissimilar cell attachment characteristics. Polyelectrolyte multilayers (PEMs) were deposited and exposed with UV light through a binary photomask to form a patterned upper crosslinked stratum where cells are not viable and a lower cytophilic stratum. Specifically, bilayers of polyacrylic acid and polyacrylamide (PAA/PAAm) were patterned (i.e., crosslinked) on top of electrostatically bound layers of biocompatible poly(allylamine hydrochloride)/poly(sodium 4-styrenesulfonate (PAH/PSS) bilayers. This work demonstrates the facile patterning of PEMs for two-dimensional cell cultures that exhibit natural phenotypic expression and long-term viability, which are significant advantages over many other cell patterning strategies.
Conference: The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (?TAS2006)
Proceedings: Proceedings of Micro Total Analysis Systems
Pages: pp. 260 - 262
Location: Tokyo, JA
Dates: November 5-9, 2006
Keywords: cell culture,coating,photo polymerization,polymer,surface modification
Research Areas: