| Author(s): |
Darwin R. Reyes-Hernandez; J. Brian Hutchison; Laurie E. Locascio; Michael Gaitan; |
| Title: |
Photopatterned Polyelectrolyte Multilayers for Cell Culture |
| Published: |
November 09, 2006 |
| Abstract: |
Patterned two-dimensional cell cultures are increasingly important for constructing bioanalytical tools for cell and molecular biology to address questions about interactions, signaling, migration, and differentiation, among others. This contribution presents a unique method for patterning layer-by- layer polymer with dissimilar cell attachment characteristics. Polyelectrolyte multilayers (PEMs) were deposited and exposed with UV light through a binary photomask to form a patterned upper crosslinked stratum where cells are not viable and a lower cytophilic stratum. Specifically, bilayers of polyacrylic acid and polyacrylamide (PAA/PAAm) were patterned (i.e., crosslinked) on top of electrostatically bound layers of biocompatible poly(allylamine hydrochloride)/poly(sodium 4-styrenesulfonate (PAH/PSS) bilayers. This work demonstrates the facile patterning of PEMs for two-dimensional cell cultures that exhibit natural phenotypic expression and long-term viability, which are significant advantages over many other cell patterning strategies. |
| Conference: |
The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (?TAS2006) |
| Proceedings: |
Proceedings of Micro Total Analysis Systems |
| Pages: |
pp. 260 - 262 |
| Location: |
Tokyo, JA |
| Dates: |
November 5-9, 2006 |
| Keywords: |
cell culture;coating;photo polymerization;polymer;surface modification |
| Research Areas: |
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