| Author(s): |
Pierre-Alain Auroux; Jayna J. Shah; James C. Booth; Mulpuri Rao; Laurie E. Locascio; Michael Gaitan; |
| Title: |
Microfluidic Method for Thermal Cycling by Microwave Dielectric Heating |
| Published: |
November 09, 2006 |
| Abstract: |
We report on temperature time domain and spectral measurements for on-chip microwave thermocycling. In particular we observe enhanced specificity of the heating of a targeted sample compared to conventional heating block-based thermocyclers. Spatial control of the temperature in the device is implemented by delivering microwave energy to specific locations in the microfluidic chip. Our device characterization shows a linear response between the rise in temperature and the applied power as predicted by theory. On-chip thermocycling by microwave dielectric heating is characterized and compared to a commercial heating block-based thermocycler. This method is applicable to any reaction where fast and accurate heat transfer is required. We are currently developing PCR-based applications based on this technique. |
| Proceedings: |
The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS) |
| Pages: |
pp. 1465 - 1467 |
| Location: |
Tokyo, JA |
| Dates: |
November 5-9, 2006 |
| Keywords: |
dielectric heating;DNA;microwave;PCR;thermocycling |
| Research Areas: |
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