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Publication Citation: Microfluidic Method for Thermal Cycling by Microwave Dielectric Heating

NIST Authors in Bold

Author(s): Pierre-Alain Auroux; Jayna J. Shah; James C. Booth; Mulpuri Rao; Laurie E. Locascio; Michael Gaitan;
Title: Microfluidic Method for Thermal Cycling by Microwave Dielectric Heating
Published: November 09, 2006
Abstract: We report on temperature time domain and spectral measurements for on-chip microwave thermocycling. In particular we observe enhanced specificity of the heating of a targeted sample compared to conventional heating block-based thermocyclers. Spatial control of the temperature in the device is implemented by delivering microwave energy to specific locations in the microfluidic chip. Our device characterization shows a linear response between the rise in temperature and the applied power as predicted by theory. On-chip thermocycling by microwave dielectric heating is characterized and compared to a commercial heating block-based thermocycler. This method is applicable to any reaction where fast and accurate heat transfer is required. We are currently developing PCR-based applications based on this technique.
Proceedings: The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS)
Pages: pp. 1465 - 1467
Location: Tokyo, JA
Dates: November 5-9, 2006
Keywords: dielectric heating,DNA,microwave,PCR,thermocycling
Research Areas: